GROUP 5: MATa Specific Promoters II
Up to today...
Since then -- we have been troubleshooting and working on getting higher concentrations and using
the correct stock plates. Several delays have occurred,and we should be close to ligating into the
iGEM vector
8/24
Restriction Enzyme Digested and the insert appers promising. Ready for sequence submission for Ste 2 promotor.
Status Report by: Alexandra McMillan
8/21
Due to low concentrations, we needed to concentrate our DNA before preceeding.
Ethanol precipitation using EtOH/NaAc and 100 ul of miniprep mix was complete of the BBa_K110015
and BBa_K11009 minipreps.
8/18
Miniprep products were < 100 ng/ul. Restriction digest. There appear to be correct
size inserts. Gel image coming. Results look promising.
8/15
DNA miniprep of BBa_K110015 and BBa_K11009 cultures
8/13
Incubated inoculations of old colony plates
By 7/19/08
We successfuly transformed both BB 15 and 9 and ran a gel that came out with faint bands.
We digested BB 9 and 15.
Date: 7/11/08
status report by Rick Carrick
Part no.: BBa_K110015,
Part Description: MFA1
Part Location (in build a genome lab):
PCR successful?; Y (on moodle somewhere)
Cloning of PCR product successful: Y
Sequencing of cloned PCR product successful:N
Joining of validated part to adjacent part(s) status: Not done
Problems to be solved: None so far
Current status of this part: This parts must be restriction enzyme digested and sequenced
next.
Date: 7/11/08
status report by Rick Carrick
Part no.: BBa_K110009
Part Description: Ste2
Part Location (in build a genome lab):
PCR successful?; Y (on moodle somewhere)
Cloning of PCR product successful: Y
Sequencing of cloned PCR product successful:N
Joining of validated part to adjacent part(s) status: Not done
Problems to be solved: None so far
Current status of this part: This part must be restriction enzyme digested and sequenced
next
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