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From 2008.igem.org
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- 13:40, 14 July 2008 (diff | hist) Team:ESBS-Strasbourg/PCR
- 13:39, 14 July 2008 (diff | hist) Team:ESBS-Strasbourg/PCR
- 13:37, 14 July 2008 (diff | hist) Team:ESBS-Strasbourg/PCR
- 13:36, 14 July 2008 (diff | hist) Team:ESBS-Strasbourg/PCR
- 13:35, 14 July 2008 (diff | hist) Team:ESBS-Strasbourg/PCR
- 12:51, 14 July 2008 (diff | hist) N Team:ESBS-Strasbourg/Ligation (New page: 1)Remarks: <br> 2)Protocol: <br> -For insert/vector ligation: <br> [http://openwetware.org/wiki/DNA_Ligation see iGEM protocol] -For intra vector ligation: <br> 5 T4 DNA ligase unit,...)
- 12:46, 14 July 2008 (diff | hist) Team:ESBS-Strasbourg/Dephosphorylation
- 12:46, 14 July 2008 (diff | hist) N Team:ESBS-Strasbourg/Dephosphorylation (New page: 1)Remarks: <br> Do the reaction directly in the RE mix <br> 2)Protocol: <br> -Add 2 unit of CIP for 2µg of vector <br> -Digestion 1h max. at 37°C <br> [[Team:ESBS-Strasbourg/Prot...)
- 12:44, 14 July 2008 (diff | hist) N Team:ESBS-Strasbourg/Agarose gel preparation (New page: 1)Remarks: <br> -For an ultrapure agarose gel preparation, see Paul he has some reference. I dunnow if we have to buy it or if some lab workers work with it here <br> 2)Protocol: <br> ...)
- 12:43, 14 July 2008 (diff | hist) Team:ESBS-Strasbourg/Restriction digestion
- 12:43, 14 July 2008 (diff | hist) N Team:ESBS-Strasbourg/Restriction digestion (New page: 1)Remarks: <br> {| class="prettytable" style="width:50%; height:50px" border="1" cellspacing="0" |- class="hintergrundfarbe5" |- | '''Name''' || '''Digestion time''' || '''Reaction t...)
- 12:42, 14 July 2008 (diff | hist) N Team:ESBS-Strasbourg/Plasmid urification (New page: 1)Remarks: <br> We can estimate the concentration of our final product, by using a spectrophotometer. The wavelength to enter is 260 nm, knowing that 1 unit correspond of a 50µg/mL conce...)
- 12:37, 14 July 2008 (diff | hist) N Team:ESBS-Strasbourg/Miniprep: plasmid amplification (New page: 1)Remarks <br> {| class="prettytable" style="width:50%; height:50px" border="1" cellspacing="0" |- class="hintergrundfarbe5" |- | '''Antibiotic''' || '''Working concentration''' |- | ...)
- 12:36, 14 July 2008 (diff | hist) Team:ESBS-Strasbourg/Purification of PCR product
- 12:34, 14 July 2008 (diff | hist) N Team:ESBS-Strasbourg/Purification of PCR product (New page: '''Purification of classic PCR product''' <br> 1)Remarks: <br> In this case we obtain linear DNA which could be purified after a Gel migration or directly after the PCR. <br> {| class="...)
- 12:32, 14 July 2008 (diff | hist) N Team:ESBS-Strasbourg/PCR (New page: '''Classic PCR''' <br> 1)Remarks: <br> -Use of the Taq polymerase <br> -Te=72°C <br> -Tm should be comprised between 55°C and 65°C. There is some exception, but in any case the Tm sho...)
- 12:30, 14 July 2008 (diff | hist) Team:ESBS-Strasbourg/Protocols (→Cloning)
- 12:25, 14 July 2008 (diff | hist) Team:ESBS-Strasbourg/Transformation Barbara (top)
- 12:23, 14 July 2008 (diff | hist) N Team:ESBS-Strasbourg/Transformation Barbara (New page: DNA in small volume (maximal 10µl) <br> + 20µl of KCM 5X <br> qsp 100µl H2O <br><br> Add 100µl of competent bacteria <br><br> Leave 20 min on ice, then 5 min at room temperature <br>...)
- 12:21, 14 July 2008 (diff | hist) Team:ESBS-Strasbourg/Protocols (→E.Coli)
- 12:19, 14 July 2008 (diff | hist) Team:ESBS-Strasbourg/Competent Bacteria (→Solutions) (top)
- 12:19, 14 July 2008 (diff | hist) Team:ESBS-Strasbourg/Competent Bacteria (→Solutions)
- 12:17, 14 July 2008 (diff | hist) N Competent Bacteria (Competent Bacteria moved to Team:ESBS-Strasbourg/Competent Bacteria) (top)
- 12:17, 14 July 2008 (diff | hist) m Team:ESBS-Strasbourg/Competent Bacteria (Competent Bacteria moved to Team:ESBS-Strasbourg/Competent Bacteria)
- 12:10, 14 July 2008 (diff | hist) Team:ESBS-Strasbourg/Competent Bacteria (→Solutions)
- 12:09, 14 July 2008 (diff | hist) N Team:ESBS-Strasbourg/Competent Bacteria (New page: =Solutions= '''KCM 5x''' <br> 100ml<br> {|cellpadding='5' |0.5M KCl |3.7 g |KCl 74,56 g/mol |- |0.15M CaCl2 |2.2 g |CaCl2 2H2O 147,02 g/mol |- |0.25M MgCl2 |5 g ...)
- 12:08, 14 July 2008 (diff | hist) N Team:ESBS-Strasbourg/Protocols (New page: ==E.Coli== '''Preparation of competent cells (Mariels/Barbaras Protocol)''' Competent Bacteria <br> '''Preparation of TOP10 chemically competent cells''' [http:/...)
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