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From 2008.igem.org
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- 20:10, 17 July 2008 (diff | hist) N Team:The University of Alberta/17 July 2008 (New page: ===Today=== *Did Minipreps of I0500 in the new vector: Now we have I0500 in a high copy plasmid! No more adding IPTG to switch it from low-copy to high-copy mode! *More Colony PCR on the s...)
- 21:26, 16 July 2008 (diff | hist) Team:The University of Alberta/15 July 2008 (→Today) (top)
- 21:22, 16 July 2008 (diff | hist) Team:The University of Alberta/16 July 2008 (→Today) (top)
- 21:19, 16 July 2008 (diff | hist) Team:The University of Alberta/16 July 2008 (→Today)
- 17:25, 16 July 2008 (diff | hist) Team:The University of Alberta/16 July 2008 (→Today)
- 17:09, 16 July 2008 (diff | hist) N Team:The University of Alberta/16 July 2008 (New page: ===Today=== *The plates of Transformants of I0500 in the new vector turned out good **Did colony PCR on two colonies to confirm they have the I0500 insert ***Streaked out both colonies **...)
- 19:42, 15 July 2008 (diff | hist) Team:The University of Alberta/15 July 2008
- 18:14, 15 July 2008 (diff | hist) Team:The University of Alberta/11 July 2008 (top)
- 18:06, 15 July 2008 (diff | hist) N Team:The University of Alberta/14 July 2008 (New page: ==Today== *Took the sequencing of Blue Ox in J61003 Transf.2 and Transf.4 up to MBSU. Should get results tomorrow. *Ligated I0500 part and the new, high-copy vector together. Will transfor...) (top)
- 17:58, 15 July 2008 (diff | hist) Team:The University of Alberta/15 July 2008 (→Today)
- 17:57, 15 July 2008 (diff | hist) N Team:The University of Alberta/15 July 2008 (New page: ===Today=== *Got sequencing back from Blue Ox in J61003 Transf.2 and Transf. 4. Both of them look good; Transf.2 looks "better" though because it has fewer mismatches. *Did PCR on Tryp aga...)
- 19:50, 9 July 2008 (diff | hist) N Team:The University of Alberta/9 July 2008 (New page: ===Today== '''Chris'''<br> *Transformed Blue Ox in J61003 ligations 2 and 4; plated these (100ul and 400ul aliquots, 4 plates total)) (top)
- 20:26, 7 July 2008 (diff | hist) Team:The University of Alberta/7 July 2008 (→=Today) (top)
- 20:25, 7 July 2008 (diff | hist) N Team:The University of Alberta/7 July 2008 (New page: ===Today== '''Chris''' *Finished off the Westerns from last week. Again, no results. This isnt a surprise though, since the staining of the gels showed there was no protein on the gels, ev...)
- 17:34, 4 July 2008 (diff | hist) Team:The University of Alberta/4 July 2008 (→=Today)
- 17:34, 4 July 2008 (diff | hist) N Team:The University of Alberta/4 July 2008 (New page: ===Today== *Our Site-Directed Mutagenesis kit has arrived! That'll be fun to do! '''Chirs''' *Continuing with the protein stuff from yesterday. Transfering the gels to nitrocellulose membr...)
- 17:30, 4 July 2008 (diff | hist) Team:The University of Alberta
- 21:03, 3 July 2008 (diff | hist) Team:The University of Alberta/3 July 2008 (top)
- 17:07, 3 July 2008 (diff | hist) Team:The University of Alberta/27 June 2008 (→Today) (top)
- 17:05, 3 July 2008 (diff | hist) Team:The University of Alberta/27 June 2008 (→Today)
- 17:05, 3 July 2008 (diff | hist) N File:N120400274 33714509 398.jpg (top)
- 16:58, 3 July 2008 (diff | hist) N Team:The University of Alberta/3 July 2008 (New page: ===Today=== '''Chris'''<br> *After staining the gels from yesterday, there were no apparent bands. Could the Ni-NTA column purifications not have worked? Will run two more gels, with the c...)
- 16:53, 3 July 2008 (diff | hist) Team:The University of Alberta/2 July 2008
- 20:04, 27 June 2008 (diff | hist) Team:The University of Alberta/27 June 2008 (→Today)
- 20:04, 27 June 2008 (diff | hist) Team:The University of Alberta/25 June 2008 (→Today) (top)
- 20:02, 27 June 2008 (diff | hist) Team:The University of Alberta/27 June 2008 (→Today)
- 16:56, 27 June 2008 (diff | hist) N Team:The University of Alberta/27 June 2008 (New page: ==Today== *The O/Ns that were set up last night turned out well. Did mini-preps on them today. *The transformations done yesterday turned out perfectly. Now [[:Image:june27_transformants|...)
- 16:48, 27 June 2008 (diff | hist) N Team:The University of Alberta/26 June 2008 (New page: ==Today== *Did Colony PCR on Tom's transformants (22, 23, 25, 35 in I0500); ran on a gel - results were sketchy: lots of bands in each lane. We think this might be due to contamination in ...) (top)
- 17:39, 25 June 2008 (diff | hist) Team:The University of Alberta/Sequencing (→June 24) (top)
- 17:35, 25 June 2008 (diff | hist) N File:June24 thio rbs 6.jpg (top)
- 17:32, 25 June 2008 (diff | hist) N File:June24 thio rbs 4.jpg (top)
- 17:32, 25 June 2008 (diff | hist) N File:June24 thio rbs 2.jpg (top)
- 17:31, 25 June 2008 (diff | hist) N File:June24 thio rbs 1.jpg (top)
- 17:30, 25 June 2008 (diff | hist) N File:June24 tetRvr.jpg (top)
- 17:29, 25 June 2008 (diff | hist) Team:The University of Alberta/Sequencing (→June 24)
- 17:28, 25 June 2008 (diff | hist) N File:June24 vf.jpg (top)
- 16:46, 25 June 2008 (diff | hist) N Team:The University Of Alberta/Sequencing/June 24/Thio RBS 4 (New page: TTCNANTTACCTATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATT TCTGGAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGAAAAATTGTGTCATCGTCAGTGCGGTACGTACT GCTATCGGTAGTTTTAACGGTTCACT...) (top)
- 16:45, 25 June 2008 (diff | hist) N Team:The University Of Alberta/Sequencing/June 24/Thio RBS 3 (New page: TANGNTTACCTATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTT CTGGAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGAGGGCCCAATTCGCCCTATAGTGAGTCGTATTACAAT TCACTGGCCGTCGTTTTACAACGTCG...) (top)
- 16:45, 25 June 2008 (diff | hist) N Team:The University Of Alberta/Sequencing/June 24/Thio RBS 2 (New page: TTACCCTATAAAAATAGGGCGGTATCACGGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTNCCCTTNAGGATGATT TCTGGAATTCGCGGCCGCTTCTAGAGAANGAGGAGAAATACTAGATGAAAAATTGTGTCATCGTCAGTGCGGTACGTACT GCTATCGGTAGTTTTAACGGTTCACT...) (top)
- 16:44, 25 June 2008 (diff | hist) N Team:The University Of Alberta/Sequencing/June 24/Thio RBS 1 (New page: CTNTGNTTACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGAT TTCTGGAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGAGGGCCCAATTCGCCCTATAGTGAGTCGTATTACA ATTCACTGGCCGTCGTTTTACAACGT...) (top)
- 16:43, 25 June 2008 (diff | hist) N Team:The University Of Alberta/Sequencing/June 24/TetR Vr (New page: CGTNCCACCGACGACGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCAGCGGCCGCTACTAGTAGCGATCTACACTAGC ACTATCAGTGTTATTAAGCTACTAAAGCGTAGTTTTCGTCGTTTGCAGCGGACCCACTTTCACATTTAAGTTGTTTTTCT AATCCGCATATGATCAATTCAAGGCC...) (top)
- 16:43, 25 June 2008 (diff | hist) N Team:The University Of Alberta/Sequencing/June 24/TetR Vf (New page: CTNCGNCTTACCTATAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCNCTNAGGATGATT TCTGGAATTCGCGGCCGCTTCTAGATGTCCAGATTAGATAAAAGTAAAGTGATTAACAGCGCATTAGAGCTGCTTAATGA GGTCGGAATCGAAGGTTTAACAACCC...) (top)
- 16:41, 25 June 2008 (diff | hist) Team:The University of Alberta/Sequencing (→June 24)
- 16:37, 25 June 2008 (diff | hist) Team:The University of Alberta/Sequencing (→June 24)
- 16:35, 25 June 2008 (diff | hist) Team:The University of Alberta/Sequencing (→June 24)
- 16:30, 25 June 2008 (diff | hist) N Team:The University of Alberta/25 June 2008 (New page: ==Today== *Got more sequencing back again today (sequencing of TetR [Chris] and Thiolase+RBS Colonies 1, 2, 4 and 6 [Tom]). Everything looks good this time. Sequences and chromatograms are...)
- 17:20, 24 June 2008 (diff | hist) Team:The University of Alberta/24 June 2008 (→Today) (top)
- 17:03, 24 June 2008 (diff | hist) Team:The University of Alberta/Sequencing
- 17:01, 24 June 2008 (diff | hist) Team:The University of Alberta/Sequencing
- 16:40, 24 June 2008 (diff | hist) N Team:The University of Alberta/24 June 2008 (New page: ==Today== *Got the sequencing results from the sequencing done Friday/Saturday/Monday. Another case of LLC (Looks Like Crap). Winnie is redoing the whole shebang (colony PCR -> gel purific...)
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