User contributions
From 2008.igem.org
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- 17:25, 24 October 2008 (diff | hist) m Team:NTU-Singapore/Modelling/Parameter/LysisaD
- 14:22, 24 October 2008 (diff | hist) User:Greenbear/sandbox/header
- 14:09, 24 October 2008 (diff | hist) User:Greenbear/sandbox/header
- 14:08, 24 October 2008 (diff | hist) User:Greenbear/sandbox/header
- 14:03, 24 October 2008 (diff | hist) User:Greenbear/sandbox/header
- 13:51, 24 October 2008 (diff | hist) User:Greenbear/sandbox/header
- 13:29, 24 October 2008 (diff | hist) Team:NTU-Singapore/Parts/Fluorescence Microscope Image
- 13:27, 24 October 2008 (diff | hist) Team:NTU-Singapore/Acknowledgements
- 13:27, 24 October 2008 (diff | hist) Team:NTU-Singapore/Parts
- 13:23, 24 October 2008 (diff | hist) Team:NTU-Singapore/Project
- 13:09, 24 October 2008 (diff | hist) User:Greenbear/sandbox/header
- 12:46, 24 October 2008 (diff | hist) N File:Newparts3 hover.jpg (top)
- 12:30, 24 October 2008 (diff | hist) User:Greenbear/sandbox/header
- 12:28, 24 October 2008 (diff | hist) User:Greenbear/sandbox/header
- 13:31, 22 October 2008 (diff | hist) Team:NTU-Singapore/Acknowledgements (→Acknowledgements)
- 14:29, 14 October 2008 (diff | hist) m User:Greenbear/sandbox/header
- 16:57, 28 September 2008 (diff | hist) N Team:NTU-Singapore/Notebook/26 September 2008 (New page: =Friday 26 September= *Collecting the plates: Detection system and Production system were suscessfully transformed into competent LuxS mutants!!!)
- 07:49, 25 September 2008 (diff | hist) m Team:NTU-Singapore/Notebook/25 September 2008
- 03:35, 25 September 2008 (diff | hist) N Team:NTU-Singapore/Notebook/25 September 2008 (New page: =Thursday 25 September= *Minipreps for LsrA-YFP, followed by digestional gel check. *Make LuxS mutants more competent. *Grow W3110 in LB plus 0.8% glucose for 4 hours, centrifuge and take ...)
- 03:32, 25 September 2008 (diff | hist) N Team:NTU-Singapore/Notebook/24 September 2008 (New page: =Wednesday 24 September= *Collect plate from Tuesday: **LsrA-YFP: 1 colony *Inoculate LsrA into 5ml LBA; inoculate W3110 and LuxS mutants into 5ml LB each.)
- 03:29, 25 September 2008 (diff | hist) N Team:NTU-Singapore/Notebook/23 September 2008 (New page: =Tuesday 23 September= 930am-6pm: *Ligation of LsrA-RBS with [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0430 YFP generator]. *Transform detection and production system into L...)
- 08:09, 22 September 2008 (diff | hist) N Team:NTU-Singapore/Notebook/22 September 2008 (New page: =Monday 22 September= *Digestional gel check for miniprepped samples: 3xYFP generator (miniprepped last Friday by LuChao). **2 samples have the correct bands: 878bp insert and 2kb vector. ...)
- 06:48, 12 September 2008 (diff | hist) Nm Team:NTU-Singapore/Notebook/12 September 2008 (New page: =Friday 12 September:= *1200am: punching and dilution into TE buffer at 50 degrees C of [http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=pSB2K3 pSB2K3] (for obtaining plasmid vector...)
- 17:46, 5 September 2008 (diff | hist) Team:NTU-Singapore/Acknowledgements
- 12:14, 2 September 2008 (diff | hist) m Team:NTU-Singapore/Notebook/2 September 2008 (→Tuesday 2 September)
- 12:13, 2 September 2008 (diff | hist) N Team:NTU-Singapore/Notebook/2 September 2008 (New page: =Tuesday 2 September= *Morning: Lu Chao: minipreps for 6 samples from Monday and prepared 1% gel. *Hung: digestional gel check.<br/>Results: **Among 2 samples of LacI-RBS-E7-Term, 1 sample...)
- 12:11, 2 September 2008 (diff | hist) N File:NTU gelcheck 0209.JPG (top)
- 12:06, 2 September 2008 (diff | hist) N Team:NTU-Singapore/Notebook/1 September 2008 (New page: =Monday 1 September= *Hung: inoculated: **2x LacI-RBS-E7-term colonies **2xLacI-RBS colonies (for minipreps) **2xLsrA-RBS-Lysis-Term (for minipreps))
- 12:04, 2 September 2008 (diff | hist) N Team:NTU-Singapore/Notebook/30 August 2008 (New page: =Saturday 30 August= *Hung: collect plates from Friday night: **The plate of LacI-RBS-E7-Term had 2 colonies. **The other 2 plates for minipreps purpose had many colonies. This also showed...)
- 11:55, 2 September 2008 (diff | hist) N Team:NTU-Singapore/Notebook/29 August 2008 (New page: =Friday 29 August= *Lu Chao: prepared new top10 competent cells (60x50ul tubes). *Hung: transformation into top10 for: **The remaining ligation mixture of LacI-RBS-E7-Term. **LacI-RBS (as ...)
- 11:50, 2 September 2008 (diff | hist) N Team:NTU-Singapore/Notebook/28 August 2008 (New page: =Thursday 28 August= *Lu Chao: inoculated 1 colony of Top10 cell into 5ml LB broth and incubated overnight at 37 degrees C. This is for the preparation of new top10 competent cells on Frid...)
- 11:47, 2 September 2008 (diff | hist) N Team:NTU-Singapore/Notebook/26 August 2008 (New page: =Tuesday 26 August= *Morning: Lu Chao: minipreps of 6 colonies inoculated on Monday and prepared 2% gel for later gel check. *Hung: digestional check:<br/>Results: **Among 4 samples of Lsr...)
- 11:40, 2 September 2008 (diff | hist) Nm Team:NTU-Singapore/Notebook/25 August 2008 (New page: =Monday 25 August= *Hung: inoculated: **2 colonies of LacI-RBS (V) - E7-Term(I) **4 colonies of LsrA-RBS (V) - Lysis-Term(I))
- 11:38, 2 September 2008 (diff | hist) Nm Team:NTU-Singapore/Notebook/23 August 2008 (New page: =Saturday 23 August= *Lu Chao: collected plates: **LacI-RBS(V)-E7-Term(I): 2 colonies **LacI-RBS(I)-E7-Term(V): no colony **LsrA-RBS(V)-Lysis-Term(I): 4 colonies)
- 11:36, 2 September 2008 (diff | hist) N File:NTU gelcheck 2608.JPG (top)
- 11:33, 2 September 2008 (diff | hist) m Team:NTU-Singapore/Notebook/22 August 2008
- 11:32, 2 September 2008 (diff | hist) N Team:NTU-Singapore/Notebook/22 August 2008 (New page: =Friday 22 August= *830am - 7pm : Hung, Lu Chao: digestion+ligation+transformation of: **LacI-RBS (vector, 2.2kb) with E7-Term (insert, 2.3kb). **LacI-RBS (insert, 250bp) with E7-Term (vec...)
- 11:31, 2 September 2008 (diff | hist) N File:NTU gel 2208.JPG (top)
- 11:54, 19 August 2008 (diff | hist) m Team:NTU-Singapore/Notebook/19 August 2008
- 11:50, 19 August 2008 (diff | hist) N File:NTU gel 19 08.JPG (E7-Term and Lysis-Term gel check) (top)
- 10:43, 19 August 2008 (diff | hist) m Team:NTU-Singapore/Notebook/19 August 2008 (→Tuesday 19 August)
- 10:10, 19 August 2008 (diff | hist) N Team:NTU-Singapore/Notebook/19 August 2008 (New page: =Tuesday 19 August= *800-930: Lu Chao: minipreps for 5xE7-term colonies and 7xlysis-term colonies. Prepare 2% gel. *1330-1400: Hung: digestion of the above plasmids with EcoRI and PstI for...)
- 10:23, 18 August 2008 (diff | hist) N Team:NTU-Singapore/Notebook/18 August 2008 (New page: =Monday 18 August= *Hung: 1545-1630: inoculate of 7xLysis-Term colonies and 5xE7-Term colonies.)
- 12:37, 17 August 2008 (diff | hist) Team:NTU-Singapore/Project/Results (→Testing)
- 12:28, 17 August 2008 (diff | hist) Team:NTU-Singapore/Project/Results (→Testing)
- 12:27, 17 August 2008 (diff | hist) Team:NTU-Singapore/Project/Results (→Testing)
- 08:13, 16 August 2008 (diff | hist) N Team:NTU-Singapore/Notebook/16 August 2008 (New page: =Saturday 16 August= *10am: Hung: collect the plates from Friday. **Plates of E7-Term had 3 colnies **Plates of Lysis-Term had 6 colonies **Plates of LsrA-Lysis had no colony :()
- 08:11, 16 August 2008 (diff | hist) N Team:NTU-Singapore/Notebook/15 August 2008 (New page: =Friday 15 August= Ligation of: #E7(I)-Terminator(V) #LsrA(V)-Lysis(I) #Lysis(V)-Terminator(I) *900-930:Hung: digestion of E7-I(E/S), Terminator-V(E/X), GFP (E/S) to compare the bands wit...)
- 17:41, 13 August 2008 (diff | hist) m Team:NTU-Singapore/Notebook/13 August 2008
- 07:32, 13 August 2008 (diff | hist) N Team:NTU-Singapore/Notebook/13 August 2008 (New page: =Wednesday 13 August= *8am: Lu Chao: minipreps for 6xLsrA-SV and 6xLysis-SV. *3pm-7pm: Hung, Lu Chao: digestional check.)
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