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User contributions
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- 03:13, 29 October 2008 (diff | hist) Team:Heidelberg/Parts/Characterization (→ColicinE1-Receiver)
- 02:55, 29 October 2008 (diff | hist) Team:Heidelberg/Notebook/team meetings (→Team Meetings)
- 02:53, 29 October 2008 (diff | hist) Team:Heidelberg/Notebook/team meetings (→Team Meetings)
- 02:48, 29 October 2008 (diff | hist) Team:Heidelberg/Notebook/Killing II/Notebook (→Perl) (top)
- 02:48, 29 October 2008 (diff | hist) N File:Convert 2 wikitable.zip (top)
- 02:48, 29 October 2008 (diff | hist) Team:Heidelberg/Notebook/Killing II/Notebook (→Perl)
- 02:47, 29 October 2008 (diff | hist) N File:Tecan scripts.zip (top)
- 02:44, 29 October 2008 (diff | hist) Team:Heidelberg/Notebook/Killing II/Notebook (→Perl)
- 02:42, 29 October 2008 (diff | hist) Team:Heidelberg/Notebook/Killing II/Notebook
- 02:41, 29 October 2008 (diff | hist) N File:Timerow maxi.m (top)
- 02:41, 29 October 2008 (diff | hist) N File:Timerow.m (top)
- 02:41, 29 October 2008 (diff | hist) N File:One plate.m (top)
- 02:40, 29 October 2008 (diff | hist) Team:Heidelberg/Notebook/Killing II/Notebook (→Matlab)
- 02:40, 29 October 2008 (diff | hist) N File:Sendertest.m (top)
- 02:40, 29 October 2008 (diff | hist) N File:Colicin char.m (top)
- 02:39, 29 October 2008 (diff | hist) N File:Colicin activity system lysis.m (top)
- 02:39, 29 October 2008 (diff | hist) N File:Colicin activity system.m (top)
- 02:39, 29 October 2008 (diff | hist) N File:Colicin one plate.m (top)
- 02:39, 29 October 2008 (diff | hist) N File:Colicin test.m (top)
- 02:39, 29 October 2008 (diff | hist) N File:One plate colicin.m (top)
- 02:38, 29 October 2008 (diff | hist) Team:Heidelberg/Notebook/Killing II/Notebook (→Colicins - Scripts)
- 02:33, 29 October 2008 (diff | hist) Team:Heidelberg/Notebook/Killing II/Notebook (→Colicins - Scripts)
- 02:32, 29 October 2008 (diff | hist) N File:Colicin activity 081020.m (Matlab script for colicin activity test data evaluation) (top)
- 02:31, 29 October 2008 (diff | hist) Team:Heidelberg/Notebook/Killing II/Notebook
- 02:17, 29 October 2008 (diff | hist) Team:Heidelberg/Parts/Characterization (→Part Characterization)
- 02:17, 29 October 2008 (diff | hist) Team:Heidelberg/Parts/Characterization (→ColicinE1-Receiver)
- 02:16, 29 October 2008 (diff | hist) Team:Heidelberg/Parts/Characterization (→Part Characterization)
- 02:15, 29 October 2008 (diff | hist) Team:Heidelberg/Notebook/Killing II/Cloning (→Killing II - Cloning Strategy)
- 02:08, 29 October 2008 (diff | hist) Team:Heidelberg/Project/Killing II (→ColicinE1)
- 02:06, 29 October 2008 (diff | hist) Team:Heidelberg/Project/Killing II (→References)
- 02:05, 29 October 2008 (diff | hist) N File:Fig 18-081028-PI eucaryotic.png (Positive PI MCF-7 cells (%) over time. E coli producing colicin E1 protein cause cell death at the earliest time point (2 h) and keep it higher then the other condition. After 3 h, the reference strain also starts to present PI positive cells that increas) (top)
- 02:04, 29 October 2008 (diff | hist) Team:Heidelberg/Project/Killing II (→Eukaryotic killing Assay (Preliminary Results))
- 02:04, 29 October 2008 (diff | hist) N File:Fig 17-081025-killerpreytest GFP 1.png (Prey-Killer system test. GFP intensity of prey strains was measured over 12 h at 37 °C. Prey cells were killed for prey-killer ratios from 1:1 up to 25:1 (blue, green, red, light blue) due to the colicin production of the killer strain. This production w) (top)
- 02:03, 29 October 2008 (diff | hist) N File:Fig 16-081028-lysis combined.png (Visualization of prey-killer system. The prey (GFP) and the killer (mCherry) strain are mixed on a 0.5 % agarosepad. Killer cells were induced with AHL before adding to the medium. The killer cell on the left picture (white arrow) is vanished after 30 min) (top)
- 02:02, 29 October 2008 (diff | hist) File:Fig 15-081027-Lysis.png (uploaded a new version of "Image:Fig 15-081027-Lysis.png": Lysis test with LuxR-ColicinE1-Receiver dependent on AHL concentrations. On the left panel the growth of a killer population induced with different concentrations of AHL is shown. At the right) (top)
- 02:02, 29 October 2008 (diff | hist) N File:Fig 15-081027-Lysis.png
- 02:02, 29 October 2008 (diff | hist) N File:Fig 14-081023-bar diagram ratios 3conc.png (Toxicity of LuxR-colicinE1receiver part for different prey-killer ratios. The graphs show GFP intensities of prey cells at t = 0 h and t = 12 h. For prey killer ratios of 1:1 all preys were killed after 12 hours even if there is no AHL present. For prey-k) (top)
- 02:01, 29 October 2008 (diff | hist) N File:Fig 13-081023-dose responsecurve 100-1.png (Dose-response curve of AHL concentration and killing efficiency of the killer strain for an prey-killer ratio of 100:1. The GFP intensities at t = 12 h are plotted against AHL concentrations (0 M – 1 nM). For AHL concentrations higher than 600 nM all pr) (top)
- 02:01, 29 October 2008 (diff | hist) N File:Fig 12-081023-P-K 100-1 growth curve.png (Results of colicin E1 toxicity tests for different AHL concentrations with a prey-killer ratio of 100:1. Top left panel: GFP intensities of prey cells treated with killer cells. Top right panel: Optical densities of prey cells treated with killer cells. ) (top)
- 02:01, 29 October 2008 (diff | hist) N File:Fig 11-081028-PCR control digestion.png (Control digestion of the final pSB1A2-receiver-colicinE1 plasmid (LuxR-colicinE1-receiver). Bright bands of ladder from bottom to top: 3000 bp, 1000 bp and 500 bp. First two bands contain approximately 5200 bp, which is the whole plasmid, cutted once by X) (top)
- 02:00, 29 October 2008 (diff | hist) N File:Fig 10-081028-PCR amplification colicins.png (PCR amplification of colicin E1 operon, the receiver part insert (T9002 without GFP) ~1088 bp and the vector pSB1A2.) (top)
- 01:59, 29 October 2008 (diff | hist) Team:Heidelberg/Project/Killing II (→Final Prey-Killer System)
- 01:58, 29 October 2008 (diff | hist) Team:Heidelberg/Project/Killing II (→ColicinE1)
- 01:57, 29 October 2008 (diff | hist) N File:Fig 9-PCR-amplification results cloning.png (PCR-amplification results of the Colicin E9 short (~1050 bp) and long (~2014 bp) insert, the receiver part insert (T9002 without GFP) ~1088 bp and the vector pSB1A2.) (top)
- 01:57, 29 October 2008 (diff | hist) N File:Fig 8-11092008-igem colicin activity sonicbath on GFP 3-1 beschriftet.png (Colicin toxicity test of lysed supernatant on E. coli TOP 10 cells harbouring BBa_I20260: The GFP intensity is related to the growth of the E. coli TOP 10 cells. These could not grew while adding supernatant of the killing strain, but grew only with super) (top)
- 01:56, 29 October 2008 (diff | hist) Team:Heidelberg/Project/Killing II (→ColicinE9)
- 01:56, 29 October 2008 (diff | hist) Team:Heidelberg/Project/Killing II (→ColicinE9)
- 01:55, 29 October 2008 (diff | hist) Team:Heidelberg/Project/Killing II (→Sender)
- 01:54, 29 October 2008 (diff | hist) N File:Fig 6-081019-sendertestresults.png (Results of sender activity test. Top left: Growth curve of sender culture. Top right: GFP/OD values of sender supernatants at t = 10 h. Bottom left: GFP/OD – AHL calibration curve. Dotted line visualized regression of an exponential growth curve. Botto) (top)
- 01:54, 29 October 2008 (diff | hist) Team:Heidelberg/Project/Killing II (→Sender)
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