Team:The University of Alberta/Parts

University of Alberta Parts Registry

All our submitted parts can be found [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=University_of_Alberta in our section of the Parts Registry]!

Plastic Project

• TetR + LVA tail[http://partsregistry.org/Part:BBa_C0040|]
• PLACIQ + ERE brick - Sequence
• Estrogen Receptor Alpha (Estrogen binding domain)
• Estrogen Receptor Alpha (DNA binding domain)
• BisdA BPA degredation bio brick - Sequence
• BisdB BPA degredation bio brick - Sequence

Parts Ordered

PDF Sequences of synthetic genes ordered 20 Jun

• Media:UA28-8genes-v2_(2).pdf

Genbank files synthetic genes ordered 20 Jun

• Media:iGEM08_BisDA.gb
• Media:iGEM08_BisDB.gb
• Media:iGEM08_ER01.gb
• Media:iGEM08_ER02.gb
• Media:iGEM08_LacIQ_ERE.gb
• Media:iGEM08_RBS_6XHis.gb
• Media:iGEM08_TDNA_MCS.gb
• Media:iGEM08_TetR_RBS_6XHis.gb

Plant Parts

• Plant CAMV 35S Promoter
• Plant Resistance Brick
  • Chloramphenicol was used
• IGEM Binary Vector
  • We constructed the IGEM binary vector using P0380 as a starting point. We removed the PUC8 Multiple cloning site from this binary vector and supplemented our own which will allow the vector to accept biobricks.
• Blue OX, Purple Russian reporter systems

Butanol Parts

BioBricks Used This Summer

• [http://partsregistry.org/Part:BBa_I725021 RBS + B-hydroxy butyryl CoA dehydrogenase] (AKA. 21)
• [http://partsregistry.org/Part:BBa_I725022 RBS + Enoyl-CoA hydratase] (AKA. 22)
• [http://partsregistry.org/Part:BBa_I725023 RBS + Butyryl CoA dehydrogenase] (AKA. 23)
• [http://partsregistry.org/Part:BBa_I725024 RBS + Butyraldehyde dehydrogenase] (AKA. 24)
• [http://partsregistry.org/Part:BBa_I725025 RBS + Butanol dehydrogenase] (AKA. 25)
• [http://partsregistry.org/Part:BBa_J61003 J61003] (without GFP)
• [http://partsregistry.org/Part:BBa_I0500 I0500]
• [http://partsregistry.org/Part:pSB1A3 pSB1A3]

Parts Submitted

• [http://partsregistry.org/Part:BBa_S04142 21 + 22] (in plasmid pSB1A3)
• [http://partsregistry.org/Part:BBa_S04145 21 + 22 + 23] (in plasmid pSB1A3)
• [http://partsregistry.org/Part:BBa_S04143 23 + 24] (in plasmid pSB1A3)
• [http://partsregistry.org/Part:BBa_S04144 24 + 25] (in plasmid pSB1A3)
• [http://partsregistry.org/Part:BBa_S04147 21 + 22 + 23 + 24 + 25] (in plasmid pSB1A3)

Parts Ordered This Summer

• ETFA (electron-transfer-flavoprotein, alpha polypeptide)
• ETFB (electron-transfer-flavoprotein, beta polypeptide)

Parts Assembled This Summer

For Protein Expression

• J61003 + 21
• J61003 + 22
• J61003 + 23
• J61003 + 24
• J61003 + 25
• I0500 + 21
• I0500 + 22
• I0500 + 23
• I0500 + 24
• I0500 + 25

For Butanol Operon

• 21 + 22 (in plasmid pSB1A3)
• 21 + 22 + 23 (in plasmid pSB1A3)
• 23 + 24 (in plasmid pSB1A3)
• 24 + 25 (in plasmid pSB1A3)
• 21 + 22 + 23 + 24 + 25 (in plasmid pSB1A3)
• ETFA + ETFB (in plasmid Puc57)