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From 2008.igem.org

Protocols

As part of our project we developed and tested a number of procedures to make BioBrick production easier, more reliable and less hassle. These are the following:

• Use of Edinbrick1 in preparation of BioBricks^TM from PCR products
• Site-directed mutagenesis using the MABEL protocol
• BABEL: BioBrick^TM assembly with blunt-ended ligation
• Bacillobricks: introduction of BioBricks into Bacillus subtilis

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