Team:The University of Alberta/2 July 2008

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Today

Chris

  • Made two SDS-PAGE gels for running the purified proteins. Stained with Coomassie and left to destain overnight.
  • Streaked out colonies used in colony PCR for Tom (see below)

Tom

  • With help of Saima and Jason, did colony PCR of 22, 23, 25, 35 in I0500 (from diliuted and concentrated Kan plates) as well as 21 and 22 in J61003. Results were ambiguous (bands of wrong sizes, bands in the water controls, etc). They will be sequenced to figure out what's what.
  • Did some dishwashing

Jason

  • Learned how to harvest Arabidopsis seeds (its pretty easy!)

Saima

  • Made lots and lots of LBA and LBK plates
  • colony PCR of 22, 23, 25, 35 in I0500

Sponsors

If you need to make long distance phone calls for your sponsors please see the computer beside the printer desktop.

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