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Talk:Team:Guelph/Notebook
From 2008.igem.org
Talk:Team:Guelph/Project From 2008.igem.org Hey guys! We haven't talked much yet, so I wanted to start us off with a list of things I hope we can do this week (Canada day week):
-Meet on Monday without Dave
-Start growing corn and Arabidopsis for GFP endophyte assays
-get team photo and member photos plus write ups for members going/done
-Get appropriate GFP labelled plasmids, E. coli nissler, and two strains of lactobacilli ready for transformation
-Find and transform the basic biobrick found here http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 into chemically competent E. coli
-Do PCR reactions with Phusion enzyme to amplify up psbA prometer, GFPuv, and teosinte branching 1 (tb1) fragment
-directly cut and ligate these PCR products into the above plasmid
Talk amonst yourselves and talk to you more about it on Wednesday. Wahed hob!
Ravid
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