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Team:University of Ottawa/25 June 2008
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Today in the Lab
Matt
- PCR Amplification
- F60, F61 primers used for PTP2 cassette. I also labelled all the primers we received that we will be using for the next few months.
- I decided to use the Kaern Lab protocol for PCR Amplification.
- The PTP2 PCR product was then run on a gel - gel results were as expected with 2275 size band.
- Glycerol Stock
- Glycerol stock of plasmids D12, S1, T123, pSS042 with LB + glycerol was performed.
Dan
- Ran gel of pSSA42, S1, D12, T123 plasmids
- This time the marker was added and expected results were obtained. SB, DA, TB, and 42B colonies were chosen for glycerol stock.
- Cleaned out DNA box
- Cleaned out all DNA that will not be used from our box
- Our names are getting complicated so I am renaming our DNA using the following legend (all other DNA was discarded)
- 597 > 7B > 7
- 598 > 8B > 8
- S1 > SB > S
- D12 > DA > D
- T123 > TB > T
- pSSA42 > 42B > 42
- Our names are getting complicated so I am renaming our DNA using the following legend (all other DNA was discarded)
- PCR amplification of S, D, T, 7 and 8
- S, D, and T plasmids were PCR amplified using primers F69 and F70
