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Team:Calgary Wetware/Results
From 2008.igem.org
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The above image depicts the results obtained when testing a couple of intermediates of System #1 (i.e. AHL production by Bad Guy #1 and signal interception by our Champion Cell). Basically, we grew a lawn of our Champion cells containing an intermediate (F2620+I13500) in tandem with our Bad Guy #1 (containing the J23039 part) in order to see whether or not our culture of Bad Guy #1 was in fact producting AHL to which our champion cells could respond. A positive AHL control was used (AHL dissolved in Methanol and Ethyl Acetate). As can been seen by the very nice green halos, our Champion cells are able to respond to AHL by producing GFP (as seen in the control quadrants) and our Bad Guy #1 cells are making AHL to which our Champion cell is also responding!
