Team:HKUSTers/Initial Proposal/Memorizer

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Yeast Two Hybrid is a well-known system that have been developed for many years. However, similar scenario also exists in E.coli, there have already been multiple two hybrid systems in E.coli. So we can adapt two hybrid system into our design. Two-Hybrid.png

  • Advantages:
  1. first adopt two hybrid system to build up bistable switch,
  2. AD and BD can have synergistic effect, a natural AND gate
  3. AD and BD form reporting system directly, which reduces intermediate steps.
  • Disadvantages: Promoter and AD/BD needs to be constructed or designed.
  1. If two hybrid alone can’t perform bistable switch, we may need to find two pairs of repressor and inducer to construct bistable switch, in that case, to have self synthesized inducer is really a problem, since it seems that AHL can be self-produced, but it will also diffuse out, which is undesirable in our model.
  2. For the AD-BD detailed design, I am still searching for past papers, I will come up with concrete molecules before June 30th.



Memorizer.png

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