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Template:Team:CityColSanFrancisco/Notebook/Notebook/CCSF Protocols
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BASIC TRANSFORMATION PROTOCOL FOR USING CCSF COMPETENT CELLS
1. Thaw competent cells on ice for 10 min.
2. Pipet 200uL cells into chilled 1.5mL rube containing 1 uL plasmid of interest
3. Mix gently by tapping
4. Incubate cells on ice in presence of plasmid for ~20 min.
5. Heat shock at 42C for 90 sec.
6. Return to ice for 2 min.
7. Plate 100uL of transformation onto LB-amp plate
