Team:UNIPV-Pavia/Protocols/Digestion

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The protocols we used


BioBrick digestion with restriction enzymes

(estimated time: 3 hours)

Materials needed:

  • Roche restriction enzymes thawed on ice
  • Roche buffer H
  • Pre-warmed at 37°C bath
  • Cut and gel-extracted vector
  • ddH2O


  • To open vectors:
    • 20 final volume