Team:UNIPV-Pavia/Protocols/Precipitation

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The protocols we used


DNA precipitation with sodium acetate

(estimated time: 1 hour)

Materials needed:

  • sodium acetate
  • absolute ethanol
  • ethanol 70%
  • previously cut plasmid


  • Add 1/10 digestion volume of sodium acetate 3 M
  • Add 2.5 digestion volume of absolute ethanol
  • Freeze at -80°C for 30 min
  • Centrifuge at 13000 rpm, 4°C for 20 min
  • decant supernatant
  • Add 50 l of 70% ethanol
  • Centrifuge at 13000 rpm, 4°C for 20 min