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Team:Edinburgh/Project/iGEM 2008 Labwork Summary/Our Primers
From 2008.igem.org
Our Primers
This is the current situation with regard to primers:
Primer sequences:
Cellulomonas fimi endogluconase A (cenA) Suitable BioBrick primers should be:
Forward: CGT gaattc gcggccgc t tctagATG TCC ACC CGC AGA ACC Reverse: CGT t actagt a TTA TTA CCA CCT GGC GTT GC
Cellulomonas fimi endogluconase B (cenB)
Suitable BioBrick primers should be:
Forward: CGT gaattc gcggccgc t tctagATG CTC CGC CAA GTC CC Reverse: CGT t actagt a TTA TTA GTT CGT CGG GAT G
Cellulomonas fimi endogluconase C (cenC)
Suitable BioBrick primers should be:
Forward: CGT t tctagATG GTT TCT CGC AGG TC Reverse: CGT ctgcag cggccgc t actagt a TTA TTA GCT GCG CGG ACG C
Cellulomonas fimi exogluconase (cex)
Suitable BioBrick primers should be:
Forward: CGT t tctagATG CCT AGG ACC ACG CC Reverse: CGT ctgcag cggccgc t actagt a TTA TTA GCC GAC CGT GCA GG
E. coli glucose-1-phosphate adenylyltransferase (glgC)
Suitable mutant primers are:
to remove the first forbidden EcoRI site:
Forward: tgttgaaaaacctgctaacc - 54°C
Reverse: aattcgataattttatcgttc -52°C
to remove the second forbidden EcoRI site:
Forward: ctcattctgcaacattgattcc - 54°C Reverse: ttcacgcgaacgcgcg - 54°C
to cause 336:Gly->Asp:
Forward: acggttgtgtgatctccg - 54°C Reverse: cggaaaccagtgagttaag- 56°C
Arabidopsis thaliana isoamylase 1 (isa1) Suitable BioBrick primers should be:
Forward: CGT t tctagATG GAT GCA ATC AAA TGC AG - 56ºC Reverse: CGT ctgcag cggccgc t actagt aTTA TTA GGG GTC TTT AAT TGG TG - 58ºC
Arabidopsis thaliana isoamylase 2 (isa2)
Suitable BioBrick primers should be:
Forward: CGT ttctagATG GCT GCG TGG TCA CC - 56ºC Reverse: CGT ctgcag cggccgc t actagt a TTA TTA AGC GGT AGT ATT GAT GG - 54ºC
Arabidopsis thaliana granule bound starch synthase 1 (GBS1)
Suitable BioBrick primers should be:
Forward: CGT gaattc gcggccgc t tctagATG GCA ACT GTG ACT GCT - 54ºC Reverse: CGT t actagt a TTA TTA CGG CGT CGC TAC G - 52ºC
