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Team:University of Ottawa/30 June 2008
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Today in the lab
Dan
- Gel of friday-saturday ligation was run
- Ligation bands are very faint
- Decided to perfrom PCR cleanup on reaction mixture
- This time I used the correct amount all reactants
- Still, gel indicated no ligation after an hour
- WIll leave in PCR machine and check tomorrow
- Digestion of 0
- Plasmid 0 containing our receptor was digested overnight with EagI in order to remove GFP which is already in the plasmid and will interfere with our system.
