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Template:Team:UC Berkeley/Notebook/CDB construction
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pBca1256-K112400
Biobricking of promoter for grpE protein PCR grpE-F and grpE-R on MG1655 (129 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI 910+2472bp, L) Product is pBca1256-K112400 ---------------------------- cb0001-grpE-F Forward Biobricking cgataGAATTCatgAGATCTccgaggtccttgttgcgaag cb0002-grpE-R Reverse Biobricking cgtagGGATCCcgcattcatctcgctaacttc
pBca1256-K112401
Biobricking of promoter for recA protein PCR recA-F and recA-R on MG1655 (317 bp, EcoR1/BamH1) Sub into pBca1256 (EcoR1/BamH1 910+2472bp, L) Product is pBca1256-K112401 ---------------------------- cb0003-recA-F Forward Biobricking cgataGAATTCatgAGATCTagagaagcctgtcggcaccg cb0004-recA-R Reverse Biobricking cgtagGGATCCcccagtgctgccgccaacgc
pBca1256-K112402
Biobricking of promoter for fabA protein PCR fabA-F and fabA-R on MG1655 (285 bp, EcoR1/BamH1) Sub into pBca1256 (EcoR1/BamH1 910+2472bp, L) Product is pBca1256-K112402 ---------------------------- cb0005-fabA-F Forward Biobricking cgataGAATTCatgAGATCTggccattacgttggctgaac cb0006-fabA-R Reverse Biobricking cgtagGGATCCgaagttaccacccgtttcgg
pBca1256-K112403
Biobricking of signal peptide (prepro) of phoA (alkaline phosphatase) PCR cb10007-F and cb10008 on MG1655 (114 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI 910+2472bp, L) Product is pBca1256-K112403 ---------------------------- cb10007-phoA-F cgataGAATTCatgAGATCTgtacatggagaaaataaaatgaaacaaagc cb10008-phoA-R cgtagGGATCCgggcttttgtcacaggggtaaac
pBca1256-K112404
Biobricking of signal peptide (prepro) of lamB protein PCR cb10009-F and cb10010 on MG1655 (132 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI 910+2472bp, L) Product is pBca1256-K112404 ---------------------------- cb10009-lamB-F cgataGAATTCatgAGATCTgaaaagcaatgactcaggagatag cb10010-lamB-R cgtagGGATCCcagccattgcctgagcagacattac
