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Template:Team:UC Berkeley/Notebook/CC construction
From 2008.igem.org
Contents |
K112500
Construction of {<HA>} basic part K112501
Wobble PCR of cc001/cc002 ( 50 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2227+ 910,L)
Product is pBca1256-K112500
--------------------------------------------
cc001 Forward Biobricking of {<HA>}
CgATAgaattcATGagatcttacccatacgacgtcccagac
cc002 Reverse biobricking of {<HA>}
ccagtggatccccagcgtagtctgggacgtcgtatggg
K112501
Construction of {<HA!} basic part K112502
Wobble PCR of cc001/cc003 ( 53 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2227+ 910,L)
Product is pBca1256-K112501
--------------------------------------------
cc001 Forward Biobricking of {<HA>}
CgATAgaattcATGagatcttacccatacgacgtcccagac
cc003 Reverse biobricking of {<HA!}
ccagtggatccttaccagcgtagtctgggacgtcgtatgggtaag
K112502
Construction of {<myc>} basic part K112503
Wobble PCR of cc004/cc005 ( 51 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2227+ 910,L)
Product is pBca1256-K112502
--------------------------------------------
cc004 Forward Biobricking of {<myc>}
CgATAgaattcATGagatctGAACAAAAACTCATCTCAGAAGAGG
cc005 Reverse Biobricking of {<myc>}
ccagtggatccCAGATCCTCTTCTGAGATGAGTTTTTG
K112503
Construction of {<myc!} basic part K112503
Wobble PCR of cc004/cc006 ( 54 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI,2227+ 910,L)
Product is pBca1256-K112503
--------------------------------------------
cc004 Forward Biobricking of {<myc>}
CgATAgaattcATGagatctGAACAAAAACTCATCTCAGAAGAGG
cc006 Reverse Biobricking of {<myc!}
ccagtggatccTTACAGATCCTCTTCTGAGATGAGTTTTTGTTC
K112504
Construction of {<barnase>} basic part K112504
PCR CC007/CC008 on barnase gene ( 361 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2227+ 910,L)
Product is pBca1256-K112504
--------------------------------------------
cc007 Forward Biobricking of {<barnase>}
CGATAgaattcATGaGATCTGCACAGG
CC008 Reverse Biobricking of {<barnase>}
CCAGTggatccTCTGATTTTTGTAAAGGTCTG
==K112505==
<pre>
Construction of {<barnase!} basic part K112505
PCR CC007/CC009 on barnase gene ( 364 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2227+ 910,L)
Product is pBca1256-K112505
--------------------------------------------
cc007 Forward Biobricking of {<barnase!}
CGATAgaattcATGaGATCTGCACAGG
CC009 Reverse Biobricking of {<barnase!}
CCAGTggatcCTTATCTGATTTTTG
K112232 (Molly's)
Construction of {rbs.barstar!} Biobrick Part K112232
PCR mea035/mea034 on Bacillus amyloliquefaciens gen (320 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112232
----------------------------------------
mea035 Forward Biobricking of {rbs.barstar!}
cgataGAATTCatgAGATCTcataagaaaggagccgcacatg
mea034 Reverse Biobricking of {rbs.barstar!}
cgttaGGATCCttaagaaagtatgatggtgatgtcgcagcc
