Team:University of Ottawa/3 July 2008

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Today in the Lab

Matt

Competent Cells
  • Ecoli integrated with ligation products were successful in growing overnight however Atcre did not show any signs of growth
  • The cells containing the ligation product will then be inoculated and left to incubate overnight for a miniprep tomorrow.
  • The Ecoli containing the dehydrogenase component failed to grow from streaking a plate yesterday,I will try and streak again today and then glycerol stock the remaining Ecoli.
    • Ligation
  • Atcre was ligated to circularize the plasmid and will be integrated into Ecoli again today.

    • Dan

    Atcre integration
  • Plate had ~4 colonies so I decided to redo integration.
    • 1,2,3,4 and S,D,T ligation
  • The concentration of these constructs was determined to be decent
  • A gel was run to verify the integrity of the constructs, S,D,T showed some contamination
  • 1,2,3,4 did not show up on the gel for unknown reasons.

    • Dan and Matt

    Integration
  • Atcre, and 1S 1D 1T 2S 2D 2T 3S 3D 3T 4S 4D 4T were integrated
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