"
Minnesota/4 July 2008
From 2008.igem.org
Revision as of 17:18, 7 July 2008 by Emartin9808 (Talk | contribs)
| Back to Notebook Home | |
| Go to Previous Day (July 3) | Go to Next Day (July 5) |
1. PCR (Polymerase Chain Reaction): PCR used to modify Lambda_cI by copying a section of the gene, adding RBS, and using RS (restriction site enzyme) to cut copied gene section at the appropriate restriction site.
2. Run PCR in Gel Electrophoresis:
| a. Used 0.8% agarose gel, TAE buffer, and ethidium bromide (interculating agent)
| b. Gel proved have correct Lambda_cI gene with 750 base pairs. Can now perform PCR purification (add buffers).
| 3. Miniprep MCherry gene
| 4. Spectrophotometry: Used to determine the concentration of DNA in solution. Spec'd Lambda_cI with RBS (PCR sample).
| |
