"
Rensselaer/7 July 2008
From 2008.igem.org
Overviewed procedure for introducing gene of interest:
pick plasmid filter paper - into buffer
primer design - insert restriction site (comparable with plasmid)
PCR (primers, DNA polymerase, dNTP's,buffer)
Digestion w/ restriction enzymes
Ligation (plasmid and insert)
introduce to bacteria (transformation)
potential problem - psuedomonas - how to get plasmid DNA?
perform PCR on solution
