Team:University of Ottawa/7 July 2008

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Today in the lab

Dan and Chris

Miniprep
  • Performed a miniprep on all the innocluated strains
    • Digestion
  • Digested miniprep constructs with HindIII and Atcre with EcoRI
    • Gel

    • Matt

    Gel
  • A gel was run for the digestion on Friday but did not turn out correctly - the correct band sizes did not appear.
  • The same digestion was performed again, this time using a little more PstI enzyme. Gel results still gave incrrect band sizes.
  • Performing an inoculation again with new colonies chosen from plates.
    • Retrieved from "http://2008.igem.org/Team:University_of_Ottawa/7_July_2008"