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Things we did today

Wetlab work

Grace

Ran RE digested ligations on 4% gel. Too low DNA concentration so no ligated products were seen.
Redid ligation and RE digest. Loaded 20 μl in 4% gel.
RE digested C0012.
Gel extraction of C0012 vector and ligated products.
Ligated C0012 vector with pnir, slr2016, and pilA.

Krystle

Extracted BBa_E0240 and BBa_I14032 using commercial grade DH5α super competent cells.

The two expected bands corresponding to the vector and the BioBrick insert are clearly visible.

Grace

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]