User:University of Washington/10 July 2008

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RP4 Conjugation

Conjugation protocol #1
·DH5α
·DH5α + S17-1 w/ RP4
·DH5α + naked RP4 DNA
·DH5α + amp resistant RFP plasmid

Lambda Red

·Synthesis tetR insert for KorA and trbA replacement

non-RP4 Conjugation

Mini Prep & UW Glycerol Stocks
·CV13(Yep13)
·pDPT51

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