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From 2008.igem.org

PCR:
PCR Settings Annealing (°C) {{{tanneal}}}° Time Elongation {{{telong}}} Number of Cycles {{{ncycles}}}	Buffer (5x) {{{buffer}}} MgCl2 10 µM {{{mgcl}}} dNTP 10 µM {{{dntp}}} Oligo F 10 µM {{{fwprimername}}} {{{fwprimerconc}}} Oligo R 10 µM {{{rvprimername}}} {{{rvprimerconc}}} Water {{{water}}} Polymerase {{{polymerase}}} DNA {{{dna}}}	Expected Size {{{size}}} Success {{{success}}} Image (click to enlarge) [[Image:{{{image}}}|30px]] Lane (0=ladder) {{{lane}}}

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