Team:ESBS-Strasbourg/Competent Bacteria

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Solutions

KCM 5x
100ml

0.5M KCl	3.7 g	KCl 74,56 g/mol
0.15M CaCl2	2.2 g	CaCl2 2H2O 147,02 g/mol
0.25M MgCl2	5 g	MgCl2 6H2O 203,30 g/mol
autoclave

TSB
95 ml

LB	about 80 ml
10% PEG (MW 3.350)	10 g
20 mM Mg++	10 ml of 200mM
autoclave
5% DMSO	5 ml

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