"
Team:ESBS-Strasbourg/11 July 2008
From 2008.igem.org
Contents |
DryLab
Modeling
WetLab
- results SD, MM and KK:
- TOP10: 5* 10^5 cfu/ug
- db3.1: 10^5 cfu/ug
- dh5alpha: 10^6 cfu/ug
- Primer
- the arrived verification primer were resuspended to give a stock solution of 50 µM
- aliquots of these primers (4x 10 µM) were produced and everything frozen (-20°C)
- Transformation
- competent Top10 cells from Invitrogen, protocol from Marias Lab: H2O (negative control), pxj (positive control), 5 BioBricks (EYFP, vector without insert, adh terminator, lacI, lamdba CI), plated on ampicillin
- competent db3.1 cells, protocol from Barbaras Lab, DNA was already in KCM and cells were just added and plated, K3 on kanamycin and A2 on ampicillin
- competent dh5alpha cells, protocol from iGEM using SOC medium: H2O (negative control), pxj (positive control), BioBrick (EYFP), plated on ampicillin
General
Quote of the day
"At least we have worked steril." (Marius)
