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Team:ESBS-Strasbourg/Protocols
From 2008.igem.org
E.Coli
Preparation of competent cells (Mariels/Barbaras Protocol) Competent Bacteria
Preparation of TOP10 chemically competent cells [http://openwetware.org/wiki/TOP10_chemically_competent_cells Igem Standard Protocol]
Transformation of competent cells (Mariels/Barbaras Protocol) Transformation
Cloning
- PCR
- Purification of PCR products
- Miniprep:Plasmid Amplification
- Plasmid Purification
- Restriction Digestion
- Agarose Gel Preparation
- Dephosphorylation
- Ligation
Solutions and buffers
SOB for the preparation of competent cells [http://openwetware.org/wiki/SOB SOB]
