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Paris/July 11
From 2008.igem.org
Cultures
Exit of the stumps stocked at -80°C and spreading (streaks) on LB boxes (without antibiotic). Boxes have been put in the steam room at 37°C at 15h45
| Code | Stump |
| DW 27 | MG1655 ΔfliA::Cm |
| NEC 283 | MG 1655 ΔfliA::FRT |
| NEC 284 | MG 1655 ΔflhD::FRT |
| DW 26 | MG 1655 ΔflgM::Cm |
| NEC 285 | MG 1655 ΔflgM::FRT |
| DW 35 | JW K1879 ΔmotA::Kan |
| NEC 288 | MG 1655 ΔmotA::FRT |
| DW 37 | JW K1068 ΔflgJ::Kan |
| NEC 287 | MG 1655 ΔflgJ::FRT |
| DW 24 | MG 1655 ΔfliC::Cm |
| NEC 286 | MG 1655 ΔfliC::FRT |
Manipulations
- Culture of DH5ɑ in 5mL of LB
- Preparation of 250mL of CaCl_2 0,1M
- Transfer of the equipment iGEM 07 on the sixth floor
