"
Team:ESBS-Strasbourg/15 July 2008
From 2008.igem.org
Contents |
DryLab
Wiki
General Layout and content (MM)
Modeling
- heavy work on the model (RS)
WetLab
Katja:
- PCR verification of the BBa_j63002(Adh_ter) clon
-> no results, no band of the fragment neither of the primers on the gel(?)
- The primer concentration has been checked; it's allright
- I will do a Mini prep tomorrow
Paul:
-PCR of Gal4 and VP16 from the plasmid given by marielle
conditions: 100ng plasmid, 2.5µL primers, pFusion+MgSO4 buffer 5µL, 5µL dNTP (2mM), pFusion 1u
=>No amplifications, no trace of primers after gel migration (1g agar in 100mL, 100V)
-second migration:
same PCR gal4 product (20 µL in gel this time)
same PCR vp16 product (20 µL in gel this time)
Vp16 for: 1.25µL in 25µL
Vp16 rev: 1.25µL in 25µL
Gal4 for: 1.25µL in 25µL
Gal4 for: 2.5µL in 25µL
Gal4 for: 3.75µL in 25µL
General
Quote of the day
"I think that our WetLab-Team goes swimming..." (MW)
