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User:University of Washington/15 July 2008
From 2008.igem.org
RP4 Conjugation
Mini prep
·pAC88
Bac-Bac conjugation protocol #1
·DH5alpha + RP4
·DH5alpha + pCS26-pac
LuxR from AraC and TetR
- Miniprepped 12 overnight culture of mutated AraC into 30 ul EB
- Ordered sequences of all 12: 8 ul (1 umol/mL) forward primer + 4 ul DNA
- Grew 4 overnight culture of D29 from Elowitz's lab
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