Team:Hawaii/Biobrick conversions

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Contents

Objectives

  • Convert GFP (BBa_E0040) into a fusion brick using site-directed mutagenesis.
  • Convert pRL1383a into a Biobrick vector by replacing its natural MCS with the Biobrick MCS

Protocol

PCR mutagenesis of GFP

Extraction of Biobrick MCS

Subcloning of GFP fusion brick and pRL1383a Biobrick vector

Results

Discussion

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