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Team:Rice University/Notebook/13 July 2008
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Sunday, July 13th
- Taylor Stevenson
- Innoculated 100mL of LB with 1.0mL of O/N cultures and grew at 37*C (for all strains excluding the VCS257 containing the temperature sensitive phage. All 5.0mL of this O/N were used to innoculate the 100mL of LB. The Resulting culture was grown at 30*C).
- All strains were grown to OD600nm of approximately 0.8 (Note: the 7524 strain never reached an appropriate optical density, however, I continued the prep with the slightly reduced titer).
- Cells were made electrocompetent following the procedure outlined here.
- Procedure resulted in 10x40uL stocks of each of the 5 strains.
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