Minnesota/17 July 2008
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1. Plasmid prep dual promoters: (1) Tet & P22mnt, (2) LacI & LAMBDAcI. |
2. Double digest of all ligations: from previous days. |
3. Sequence L5, L6-L9 |
4. Re-transform RFP, TetR promoter, terminator because no cell growth on plates. |
5. Discuss problems with sequences |
6. Model: ?? Why GFP output is low??? |