| 1. Plasmid prep dual promoters: (1) Tet & P22mnt, (2) LacI & LAMBDAcI.
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| 2. Model: Figure out why have such a low GFP output when run model.
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| 3. Sequence L5, L6-L9
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| 4. Re-transform RFP, TetR promoter, terminator because no cell growth on plates.
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| 5. Discuss problems with sequences
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6. Double digest of all ligations: from previous days. Do a double digest to cut the components linearly, which will then be able to run through a gel to check if correct DNA is present. Follow the table below:
| Parts | 10x Buffer | BSA | H20 | DNA | RE 1 | RE 2
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| L2a, LAMBDAcI:Term | 5.0uL | 0.5uL | 32.5uL | 10.0uL | EcoRI | Xba1
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