"
Team:The University of Alberta/22 July 2008
From 2008.igem.org
Mike added links to two protein complementation papers in the Project/Journals section of the Wiki. One of these uses a Vibrio transcription factor (ToxR) fragment and Vibrio promoter in E. coli, and the other uses B-galactosidase in E. coli. Can anyone figure out how to make the appropriate BioBricks to fuse these to the existing ER LBD BioBrick? We should be able to amplify the B-galactosidase fragments from E. coli, but we will need to synthesize the Vibrio fragments and promoter. We will also need to order a lacZ deletion strain of E. coli (listed in the paper) if we don't already have something like it.
Can anyone commit to designing these primers and synthetic constructs (ASAP?)
