EPF-Lausanne/21 July 2008

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Transformation of Friday : Results : The control worked well. One of the part, who had been treated with elution buffer instead of TE, had a few colonies (about 10). This part, treated with TE, had also 4-5 colonies but all at the same place... The other part had no colonies, whichever buffer we had used.


We tried to make a new transformation with 2 random parts in TE and in an other buffer(elution buffer from a miniprep kit).. We followed the iGEM protocol, but this time, we incubated the DNA in the buffer for 20 minutes at 42degrees.