Minnesota/22 July 2008
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1. Pick Colonies from plates made 07-21-2008Start cultures. |
2. Plasmid prep: Prep RFP, YFP, GFP, TetR promoter, Terminator. Follow QIA miniprep procedure --> 1hr long. |
3. Double digest: LacI Promoter, p22 cII gene, RFP, YFP, BV (dual promoters, GFP:Term already d.dig.). |