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Team:University of Ottawa/19 July 2008
From 2008.igem.org
Contents |
Today in the lab
Dan
- Ligation
- Spiked my overnight ligation with 1 uL ATP and 1 uL enzyme.
- Ligation of Atcre
- Began ligation of Chris's Atcre component in ~50 uL reaction
Matt
- Inoculation
- Today I took out the inoculated bacteria from Friday and put them in the -4 fridge (PTP2). All of the bacteria grew and the control was clean.
- I also inoculated both BY4742 WT and YPH500 WT for Katy for a Mass Spec test.
- PCR
- I also performed a PCR cleanup of the 600/01 plasmids after I confirmed them on a Gel. These plasmids will now be ready for integration next week.
