"
Team:Paris/Notebook/Freezer
From 2008.igem.org
PCR products
| PCR products | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Number | Name | Oligo F | Oligo R | Matrix | Length | Comments | Product Left ? | []ng/µL | Date |
| [[ ]] | |||||||||
| [[ ]] | |||||||||
Plasmids: MiniPrep Products
| Plasmids: MiniPrep Products | |||||
|---|---|---|---|---|---|
| Number | Name | Biobricks | Description | Products in use | Date |
| MP1 | [http://partsregistry.org/Part:BBa_J23100 pJ23100] in [http://partsregistry.org/Part:BBa_J61002 J61002] | 
| Strong constitutive promoter in J61002 (clones 1 + 2) | 2007 | |
| MP2 | [http://partsregistry.org/Part:BBa_J23107 pJ23107] in [http://partsregistry.org/Part:BBa_J61002 J61002] |
| Medium constitutive promoter in J61002 (clones 1 + 2) | MP2.2 | 2007 |
| MP3 | [http://partsregistry.org/Part:BBa_B0015 B0015] in [http://partsregistry.org/Part:BBa_SB1AK3 pSB1AK3] | 
| Double terminator (clones 1 + 2) | MP3.3, MP3.4 | 2007 |
| MP4 | [http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:BBa_SB2K3 pSB2K3] |
| AraC pBAD (clones 1 + 2) | 2007 | |
| MP5 | [http://partsregistry.org/Part:BBa_B0030 B0030] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] | 
| Strong RBS (clones 1 + 2) | 2007 | |
| MP6 | [http://partsregistry.org/Part:BBa_E0422 E0422] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] | 
| ECFP (RBS+LVA+Term) (clones 1 + 2) | MP6'.1, MP6'.2 | 2007 |
| MP8 | [http://partsregistry.org/Part:BBa_E0840 E0840] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] |
| gfp tri-part; strong rbs (clones 1 + 2) | 2007 | |
| Plasmids: MiniPrep "in preparation" | |||||
|---|---|---|---|---|---|
| Number | Name | Biobricks | Description | Products in use | Date |
| [http://partsregistry.org/Part:BBa_B0034 B0034] |
| RBS | 2008 | ||
| [http://partsregistry.org/Part:BBa_C0040 C0040] | 
| tetR repressor | 2008 | ||
| [http://partsregistry.org/Part:BBa_C0079 C0079] |
| lasR activator | 2008 | ||
| [http://partsregistry.org/Part:BBa_C0179 C0179] |
| lasR activator | 2007 | ||
| [http://partsregistry.org/Part:BBa_E0030 E0030] |
| YFP | 2008 | ||
| [http://partsregistry.org/Part:BBa_E0040 E0040] |
| GFP | 2008 | ||
| [http://partsregistry.org/Part:BBa_E1010 E1010] |
| mRFP | 2008 | ||
| [http://partsregistry.org/Part:BBa_J23101 J23101] |
| constitutive promoter member family | 2008 | ||
| [http://partsregistry.org/Part:BBa_J23109 J23109] |
| constitutive promoter member family | 2008 | ||
| [http://partsregistry.org/Part:BBa_R0040 R0040] |
| tetR repressible promoter | 2008 | ||
| [http://partsregistry.org/Part:BBa_R0079 R0079] |
| promoter (lasr & PAI regulated) | 2008 | ||
| [http://partsregistry.org/Part:BBa_S03154 S03154] |
| intermediate construction | 2008 | ||
| [http://partsregistry.org/Part:BBa_S03879 S03879] |
| intermediate construction | 2008 | ||
Digestion Products
PURPLE = Vector
GREEN = Insert
| Digestion Products | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Number | Product Name | Matrix Name | Enzyme 1 | Enzyme 2 | Size | Description | Product Left | []ng/µL | Date |
| D1 | <bbpart>pJ23100</bbpart> | <bbpart>pJ23100</bbpart> | Spe1 | Pst1 | 2096 | Vector with the J23100 promoter ready for insertion of a RBS/CDS insert | Ø (24/09/07) | 34 | July 17 |
| D2 | <bbpart>mRFP dblTer</bbpart> | <bbpart>pJ23100</bbpart> | Spe1 | Pst1 | 887 | 27 | July 17 | ||
