Alberta NINT/21 July 2008

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lab work (JD):

         Replated cells gave us more cells on the weekend:
                  K102031: 4
                  K102021: 4
                  Negative: 0
         Wayne made O/N of these cells as well.
         Mini-preps of K102031.1-K102031.4 and K102021.1-K102021.4
         Sequencing reaction for all 8 and dropped off at MBSU

lab work (SD):

            Results from 18/07/08 Transformation:
                        1/100      4/100      1/50       2/50
            LB+amp50        8        20         1          10
            LB+kan35        6        15         0           8
            LB+amp50+kan35  0         0         0           0
            The concentrations of the DNA for the plasmids used must be checked as these are lower counts
            than expected.             
            Inoculated a 96-well plate with overnight cultures in preparation for the lacZ assay.

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