Team:KULeuven/23 July 2008

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Contents

Lab Work

Wet Lab

Stefanie and I prepared wet cultures from the plates with the cells we received from iGEM. We also started on the glycerol stock. Tomorrow we'll continue and make a glycerol stock of all the parts. The other team started with restriction and ligation of the output.

Dry Lab

One big question: is the stop codon in the scar a problem in linking a tag and a protein coding region? The BioBricks of [http://partsregistry.org/wiki/index.php?title=Part:BBa_I712043 Ljubljana] prove not, but is this correct?

Modeling

Modeling wiki has been updated, Output, Cell Death, Inverter, Memory, Filter, Pulse Generator(still need for a parameter check) have been revisited, added graphs of both CellDesigner and Matlab. A pdf file containing the ODE equations and more was made.

Remarks