Minnesota/23 July 2008
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1. Maxiprep the 2 50.0mL cultures of Base Vector: Maxi-prep (just like the QIA prep but with larger amount of solution) the base vector cultures. |
2. Spec the base vector products: Using spectrophotometry, find the concentration of DNA in the base vectors grown O/N. Results: 0% concentration of DNA. We are screwed. |