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Team:BCCS-Bristol/Calendar-Notebook/1 July 2008
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| Media for Plates | 0.3% 400ml | 0.15% 150ml | 0.5% 150ml |
| Bactotryptone | 4g | 1.5g | 1.5g |
| NaCl | 2g | 0.75g | 0.75g |
| BactoAgar | 1.2g | 0.225g | 0.75g |
in addition did a streak out for master colonies of MG1655 in LB from table below overnight at 37oC
| 10ml LB | ' |
| Yeast Extract | 0.05 |
| Tryptone | 0.1 |
| Nacl | 0.1 |
SOC medium in preparation for Biobrick transformation this consisted of
| Trypton | 2g |
| Yeast Extract | 0.5g |
| NaCl | 0.05g |
in 95ml of water, then autoclaved. after this add this:
| MgCl2 | 0.5ml 2M solution |
| Glucose | 2ml 1M |
then freeze at -20oC. this solution is from Molecular Cloning 3 A.2 SOB/SOC Medium.
