Team:The University of Alberta/24 July 2008
From 2008.igem.org
System 2
Today we have taken the ligation from yesterday and transformed it as well as digested it.
- if all goes well the digested product should be B0034+TETR
- Column Purified the digested product.
- we will then ligate the B0034+TETR with LacIQERE giving us a complete system 2.
- Digested pTet with Spe/Pst.
- Digested RFP with Xba/Pst.