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Team:Johns Hopkins/Notebook/GROUP 1: Fluorescent Proteins
From 2008.igem.org
GROUP 1: Fluorescent Proteins
Date: July 1 2008 status report by: Ingrid (work done by James) Part no.: BBa_K110017 Part Description: yESapphire Part Location (in build a genome lab): In James and Jasper's PCR product Box, Stainless Steel 4 degree PCR successful?; Yes Cloning of PCR product successful: Y/N Sequencing of cloned PCR product successful: Not done Joining of validated part to adjacent part(s) status: Not done Current status of this part: This part is being Sequenced
Date: July 1 2008 status report by: Ingrid (work done by James) Part no.: BBa_K110010 Part Description: yESapphire Part Location (in build a genome lab): In James and Jasper's PCR product Box, Stainless Steel 4 degree PCR successful?; Yes Cloning of PCR product successful: Y/N Sequencing of cloned PCR product successful: No Joining of validated part to adjacent part(s) status: Not done Current status of this part: This part is being sequenced
Date: July 17 2008 status report by: Ingrid Part no.: BBa_K110018 Part Description: mCherry: Part Location (in build a genome lab): In silver fridge by door PCR successful?; Yes [http://www.jhu.edu/iGEM/Group1:FluorescentProteins/2008-7-22.PCR%20Products%20of%20mCherry%20and%20vYFP.Ingrid%20&%20Tejas.html PCR Products of mCherry and vYFP] Cloning of PCR product successful: Inconclusive [http://www.jhu.edu/iGEM/Group1:FluorescentProteins/2008-7-22.Venus%20YFP%20and%20mCherry%20Miniprep%20check%20via%20digest.Ingrid.html Venus YFP and mCherry Miniprep check via digest] Sequencing of cloned PCR product successful: Not done Joining of validated part to adjacent part(s) status: Not done Problems to be solved: Had some extra unknown products, with unknown bands in the gel Current status of this part: Done with touchdown PCR and gel. Next step is to clone product.
Date: July 17 2008 status report by: Ingrid Part no.: BBa_K110019 Part Description: mCherry: Part Location (in build a genome lab): In silver fridge by door PCR successful?; Yes [http://www.jhu.edu/iGEM/Group1:FluorescentProteins/2008-7-22.PCR%20Products%20of%20mCherry%20and%20vYFP.Ingrid%20&%20Tejas.html PCR Products of mCherry and vYFP] Cloning of PCR product successful: Inconclusive [http://www.jhu.edu/iGEM/Group1:FluorescentProteins/2008-7-22.Venus%20YFP%20and%20mCherry%20Miniprep%20check%20via%20digest.Ingrid.html Venus YFP and mCherry Miniprep check via digest] Sequencing of cloned PCR product successful: No Joining of validated part to adjacent part(s) status: Not done Problems to be solved: Need to figure out if gel after digest shows correct products
Date: July 17 2008 status report by: Ingrid Part no.: BBa_K110020 Part Description: Venus Enhanced YFP Part Location (in build a genome lab): In silver fridge by door PCR successful?; Yes [http://www.jhu.edu/iGEM/Group1:FluorescentProteins/2008-7-22.PCR%20Products%20of%20mCherry%20and%20vYFP.Ingrid%20&%20Tejas.html PCR Products of mCherry and vYFP] Cloning of PCR product successful: inconclusive [http://www.jhu.edu/iGEM/Group1:FluorescentProteins/2008-7-22.Venus%20YFP%20and%20mCherry%20Miniprep%20check%20via%20digest.Ingrid.html Venus YFP and mCherry Miniprep check via digest] Sequencing of cloned PCR product successful: No Joining of validated part to adjacent part(s) status: Not done Problems to be solved: Had some extra unknown products, with unknown bands in the gel Current status of this part: Done with touchdown PCR and gel. Next step is to clone product.
Date: July 17 2008 status report by: Ingrid Part no.: BBa_K110021 Part Description: Venus Enhanced YFP Part Location (in build a genome lab): In silver fridge by door PCR successful?; Yes [http://www.jhu.edu/iGEM/Group1:FluorescentProteins/2008-7-22.PCR%20Products%20of%20mCherry%20and%20vYFP.Ingrid%20&%20Tejas.html PCR Products of mCherry and vYFP] Cloning of PCR product successful: Inconclusive [http://www.jhu.edu/iGEM/Group1:FluorescentProteins/2008-7-22.Venus%20YFP%20and%20mCherry%20Miniprep%20check%20via%20digest.Ingrid.html Venus YFP and mCherry Miniprep check via digest] Sequencing of cloned PCR product successful: No Joining of validated part to adjacent part(s) status: Not done Problems to be solved: Had some extra unknown products, with unknown bands in the gel Current status of this part: Done with touchdown PCR and gel. Next step is to clone product.
