"

 

From 2008.igem.org

Results of extraction : Electrophoresis

conditions :

• 10µl of ladder 1 kb
• 2µl of extraction added with 2µl of loading Dye 6x
• migration ~30min at 100W
• Gel 1, 2, 3, 4, 5 = 0.8%
• Gel 6 = 1,5%

gel1 gel2 gel3 gel4

gel5 gel6

Name	BioBrick	Tube N°	Enz 1	Enz 2	Obs	Exp Size Matrix	Exp Size BB	Mea Size Matrix	Mea Size BB	Gel	Band
D100	B0034	1	XbaI	PstI	BI	2057 pb	34 pb	1800 pb	40 pb	6	2
		2								6	3
		3	EcoRI	XbaI	FV	2076 pb	15 pb	2000 pb	-	1	2
		4	SpeI	PstI	BV	2077 pb	14 pb	2000 pb	-	1	3
		5								1	4
D101	J23101	1	SpeI	PstI	BV	2100 pb	883 pb	2000 pb	700 pb	1	5
		2								1	6
D102	J23109	1	SpeI	PstI	BV	2100 pb	883 pb	2000 pb	700 pb	1	7
		2								1	8
D103	R0079	1	SpeI	PstI	BV	2222 pb	14 pb	2000 pb	-	1	9
		2								1	10
D104	R0040	1	SpeI	PstI	BV	2119 pb	14 pb	2000 pb	-	1	11
		2								1	12
D105	S03154	1	SpeI	PstI	BV	2750 pb	14 pb	3000 pb	-	2	5
		2	XbaI	PstI	BI	2057 pb	707 pb	2000 pb	700 pb	2	2
		3								2	3
		4	EcoRI	SpeI	FI	2056 pb	708 pb	2000 pb	700 pb	2	4
D106	S03879	1	SpeI	PstI	BV	2768 pb	14 pb	2700 pb	-	2	6
		2	XbaI	PstI	BI	2057 pb	725 pb	2000 pb	700 pb	2	7
		3								2	8
		4	EcoRI	SpeI	FI	2756 pb	726 pb	3000 pb	700 pb	2	9
D107	C0079	1	EcoRI	SpeI	FI	4402 pb	779 pb	5000 pb	800 pb	2	10
		2	XbaI	PstI	BI	4003 pb	778 pb	5000 pb	800 pb	2	11
D108	C0179	1	EcoRI	SpeI	FI	4402 pb	746 pb	2000 pb	800 pb	2	12
		2	XbaI	PstI	BI	4403 pb	745 pb	2000 pb	800 pb	3	2
D109	E0030	1	EcoRI	SpeI	FI	3166 pb	746 pb	3000 pb	700 pb	3	3
		2	XbaI	PstI	BI	3167 pb	745 pb	3000 pb	700 pb	3	4
D110	E0040	1	EcoRI	SpeI	FI	2056 pb	743 pb	2000 pb	700 pb	3	5
		2	XbaI	PstI	BI	2057 pb	742 pb	2000 pb	700 pb	3	6
D111	E1010	1	EcoRI	SpeI	FI	4402 pb	704 pb	5000 pb	700 pb	3	7
		2	XbaI	PstI	BI	4403 pb	703 pb	5000 pb	700 pb	3	8
D116	J23100	1	SpeI	PstI	BV	2100 pb	883 pb	2000 pb	900 pb	3	9
		2								3	10
D117	J23107	1	SpeI	PstI	BV	2100 pb	883 pb	2000 pb	900 pb	3	11
		2								3	12
D118	B0015	1	EcoRI	XbaI	FV	3303 pb	15 pb	3000 pb	-	4	2
		2								4	3
D119	I0500	1	SpeI	PstI	FV	5621 pb	14 pb	6000 pb	-	4	4
		2								4	5
D120	B0030	1	XbaI	PstI	BI	2057 pb	37 pb	2000 pb	50 pb	6	4
		2								6	5
		3	EcoRI	XbaI	FV	2079 pb	15 pb	2200 pb	-	4	6
		4	SpeI	PstI	BV	2080 pb	14 pb	2000 pb	-	4	7
		5								4	8
D121	E0422	1	XbaI	PstI	BI	2057 pb	939 pb	2000 pb	1100 pb	4	9
		2								4	10
		3								4	11
D122	E0	1	XbaI	PstI	BI	2057 pb	900 pb	2000 pb	1000 pb	4	12
		2						2000 pb	900 pb	5	2
		3								5	3

==> conclusion : all the digestion have succeed.....GREAT !

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