Team:ESBS-Strasbourg/28 July 2008

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Contents

DryLab

Modeling

WetLab

Sandra

  • Hybridization of the oligos for the linker sequence and the MCS
  • Blunting of the linker and Phenol-Chloroform-Precipitation

Paul -Digestion of lacI/lexA/tetR/gal4/plasmid death, by EcoR1 and Spe1
-gel migration and purification of those product
-ligation overnight at 21°C

=>Cut with the wrong enzymes; now use of Spe1 and Xba1

General

Sandra

  • Slides for presentation


Marius

  • Presentation (Layout,Assembly)

Quote of the day

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