Team:ESBS-Strasbourg/Concentrations Measurements

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Dna concentrations measurements

by spectrophotometer

step1: Prepare your sample; 2µL of the solution 

                           400µL Water

step2: put lambda=260nm

step3: do the blank with the same water you used for dilution

step4: Do the conversion absorbance->concentrations

1DO = 50µgDNA/mL = 50ng/µL

=>Multiply your DO per 50, and then per 200 for the dilution factor. Then you have your DNA concentrations in ng/µL.

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